Methods to prevent PCR amplification of DNA from non-viable virus were not successful for infectious laryngotracheitis virus
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چکیده
منابع مشابه
Detection of infectious laryngotracheitis virus (Gallid herpesvirus-1) from clinically infected chickens in Egypt by different diagnostic methods
Infectious laryngotracheitis (ILT) disease is an acute highly contagious viral disease leading to massive economic losses to the national poultry industry. This study aimed to identify the most accurate and rapid diagnostic methods to rescue layer poultry farms from intense outbreaks in Egypt. Fifty pathological specimens were collected and subjected to virus isolation (VI), histopathology, dir...
متن کاملMolecular biology of avian infectious laryngotracheitis virus.
Infectious laryngotracheitis virus (ILTV) is an alphaherpesvirus that causes an economically important chicken disease, which results in delayed growth, reduced egg production, and also frequently in death of the animals. After acute infection of the upper respiratory tract, the virus can establish latency in the central nervous system, and subsequent reactivations can lead to infection of naiv...
متن کاملDNA sequence and transcriptional analysis of the UL1 to UL5 gene cluster of infectious laryngotracheitis virus.
We have cloned and sequenced the KpnI L and M restriction fragments of infectious laryngotracheitis virus (ILTV, gallid herpesvirus 1) DNA, which are localized adjacently at the right end of the unique long region of the genome. Within the 6930 bp DNA sequence six complete open reading frames (ORFs) were identified. The predicted amino acid sequences of four of them exhibit significant homologi...
متن کاملReplication Kinetic of Infectious Laryngotracheitis Virus in Embryonic Chicken Neural Stem Cell
Background and Aims: Infectious laryngotracheitis virus (ILTV) infection causes a highly contagious respiratory disease in poultry leads to significant economic losses in the poultry industry. The genomic and antigenic characteristics between virulent and vaccine strains of ILTV are very similar. The ability of embryonated chicken neural stem (CNS) cell for differential diagnosis of ILTV strain...
متن کاملDetection of Infectious Laryngotracheitis Virus by Real-Time PCR in Naturally and Experimentally Infected Chickens
Infectious laryngotracheitis (ILT) is an acute, highly contagious upper-respiratory infectious disease of chickens. In this study, a real-time PCR method was developed for fast and accurate detection and quantitation of ILTV DNA of chickens experimentally infected with ILTV strain LJS09 and naturally infected chickens. The detection lower limit of the assay was 10 copies of DNA. There were no c...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2020
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0232571